The flow cytometer Diaries
The flow cytometer Diaries
Blog Article
At that time, the early indications have been dismissed as particles, however the retrospective evaluation shown shared properties While using the problematic cells in society. Tracing the an infection served the lab establish more laboratory processes for screening and security of assay-critical mobile traces.
No matter whether you’re a rookie or a professional, examine our sources to advance your analysis and expertise in flow cytometry.
(B) RAW 264.7 macrophages are often annotated inaccurately with regard to particle count due to their complexity and noncircular borders. The number of singlets accessible for Examination raises once the user trains and employs a far more accurate product.
Explore new insights into your experimental data by visualizing populations utilizing mixed fluorescence and morphology information. Our software program interface wizards allow downsampling, concatenation (merged information) and dimensionality resources which are created into your program for an intuitive, streamlined information analysis expertise.
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Additionally, it is illuminating to discover the number of doublets slip as a result of conventional singlet gates, and terrific to verify the standing of sudden double-beneficial functions in my Assessment."
Pair your Attune flow cytometer with the autosampler for elevated workflow effectiveness, and increase robotic integration for maximum throughput.
Our scientists will layout panels for you as often times you will need—gratis, no products acquire wanted.
The Attune NxT cytometer would be the reliable workhorse instrument your lab can rely upon for regular and trustworthy general performance. The Attune CytPix product includes a significant-speed brightfield digicam and AI-driven graphic Evaluation computer software, with modern upgrades such as device Mastering applications and an intuitive user interface, enabling Improved details precision, precision, and more rapidly insights into sample biology.
Perhaps most importantly, these activities incorporate cells of clearly distinctive phenotypes which could lead to incorrect conclusions with regards to double favourable occasions (especially in unusual populations).
Audio waves empower precise mobile alignment, resulting in Outstanding info good quality and ~10X speedier sample flow fees.
Software Take note: Automatic graphic Assessment lessens consumer-to-person variability in flow cytometry gating procedures
Designed-in dimensionality reduction resources enhanced resolution into populations. Fluorescently gated populations back-gated onto UMAP plot to substantiate envisioned populace resolution in lysed total human blood. Consumer can watch events from Every cluster for visual confirmation of cell phenotypes.
No wash/no lyse leukocyte Assessment with imaging. Cells were obtained from 24-hour-old blood by dilution in one mM EDTA ( Expert UX/UI enables buyers to prepare AI versions for expanded programs. (A) The “Teach Model” aspect supplies annotation equipment and true-time design instruction accuracy.
At the guts of every Attune Flow Cytometer lies our innovative acoustic concentrating technology, which utilizes audio waves to meticulously align cells throughout the flow mobile. This revolutionary approach surpasses regular hydrodynamic solutions, causing a lot quicker read more processing speeds, Extraordinary details high quality, and elevated sample throughput.
Because the mobile line experienced Formerly been analyzed on the Attune CytPix Flow Cytometer, the researchers went back to the photographs and ended up in a position to document the microbial an infection at the least 5 days previously.
The Attune CytPix Flow Cytometer will allow customers to visualize samples all through acquisition, validate the accuracy of gating procedures, and guidance outcomes with dependable documentation for publication reasons.
Below, we exhibit that by gating procedures utilizing image-based quantitative parameters can distinguish interacting cells from coincident activities to a lot more properly assess interacting cells.